8BU0
Crystal structure of an 8 repeat consensus TPR superhelix with calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 67.428, 67.428, 72.613 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.550 - 1.400 |
R-factor | 0.18328 |
Rwork | 0.183 |
R-free | 0.19734 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.720 |
Data reduction software | MOSFLM (7.4.0) |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.400 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmeas | 0.080 | 1.905 |
Rpim | 0.018 | 0.441 |
Total number of observations | 676928 | 98822 |
Number of reflections | 37860 | 5477 |
<I/σ(I)> | 14.2 | 1.8 |
Completeness [%] | 99.5 | |
Redundancy | 17.9 | 18 |
CC(1/2) | 0.999 | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 100 mM NaOAc, pH 5.5, 20 mM CaCl2, 30% MPD |