8BTY
Structure of the active form of ScpB, the C5a-peptidase from Streptococcus agalactiae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 167.152, 167.152, 141.485 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.840 - 1.700 |
R-factor | 0.1895 |
Rwork | 0.188 |
R-free | 0.21610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eif |
RMSD bond length | 0.006 |
RMSD bond angle | 0.773 |
Data reduction software | XDS (January 31, 2020) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.253 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmeas | 0.212 | 5.659 |
Rpim | 0.033 | 0.895 |
Number of reflections | 127120 | 12428 |
<I/σ(I)> | 16.63 | 0.34 |
Completeness [%] | 99.9 | 99.01 |
Redundancy | 40 | 39.6 |
CC(1/2) | 1.000 | 0.237 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 2.5 M ammonium sulfate, 0.1 M Hepes/KOH pH 7.8 |