8BTJ
Murine cytomegalovirus protein M35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.090, 130.711, 67.827 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 57.950 - 1.940 |
| R-factor | 0.1765 |
| Rwork | 0.174 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.242 |
| Data reduction software | autoPROC |
| Data scaling software | AutoProcess |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20-4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.520 | 2.151 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rpim | 0.047 | 0.488 |
| Number of reflections | 50243 | 2513 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 92.0 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.999 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M Na2 Malon, 0.1 M HEPES pH 7.0, 0.5 % (v/v) Jeffamine ED-2001 protein conc: 3.6mg/ml cryoprotectant: 12 (v/v) (2R,3R) -(-)-2,3-Butanediol |
