8BT9
Crystal structure of SlpA domain II (aa 201-310), domain that is involved in the self-assembly and dimerization of the S-layer from Lactobacillus acidophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033230 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 137.690, 137.690, 51.301 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.124 - 2.100 |
Rwork | 0.189 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.498 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.124 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.012 | 0.485 |
Number of reflections | 32853 | 3272 |
<I/σ(I)> | 23.76 | 1.57 |
Completeness [%] | 100.0 | 99.97 |
Redundancy | 2 | 2 |
CC(1/2) | 1.000 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Condition: 1.0 M Sodium citrate tribasic dihydrate, 0.1 M MES, pH 6.5 Protein: 10 mg/ml in 150 mM NaCl, 25 mM HEPES, pH 7.0 0.5 ul protein mixed with 0.5 ul condition |