8BT6
Crystal structure of Toxoplasma gondii glideosome-associated connector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.399, 146.661, 185.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.330 - 2.330 |
| R-factor | 0.2315 |
| Rwork | 0.229 |
| R-free | 0.27180 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 141753 | 10315 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.5 | |
| CC(1/2) | 1.000 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Tris, pH 8.4, 1.4 M potassium sodium tartrate, 50 mM magnesium acetate |
