8BT4

Ribonucleotide Reductase class Ie R2 from Mesoplasma florum, radical-lost ground state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	175.503, 53.369, 79.030
Unit cell angles	90.00, 108.38, 90.00

Refinement procedure

Resolution	48.620 - 1.350
R-factor	0.1444
Rwork	0.143
R-free	0.16400
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.743
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.620	1.398
High resolution limit [Å]	1.350	1.350
Rmeas	0.053	0.939
Number of reflections	144603	13258
<I/σ(I)>	14.72	1.88
Completeness [%]	94.9	87.47
Redundancy	4.3	4.3
CC(1/2)	0.999	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	175 mM calcium acetate, 100 mM ammonium sulphate and 20% (w/v) polyethylene glycol (PEG) 3350