8BT1
YdaT transcription regulator (CII functional analog)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 |
Unit cell lengths | 45.570, 64.256, 63.910 |
Unit cell angles | 66.83, 89.36, 77.67 |
Refinement procedure
Resolution | 44.373 - 2.398 |
R-factor | 0.213955987448 |
Rwork | 0.211 |
R-free | 0.26582 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.959 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.373 | 2.520 |
High resolution limit [Å] | 2.390 | 2.400 |
Rmerge | 0.082 | 0.386 |
Rmeas | 0.106 | 0.552 |
Number of reflections | 19698 | 993 |
<I/σ(I)> | 6 | |
Completeness [%] | 77.4 | |
Redundancy | 2.3325 | |
CC(1/2) | 0.990 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 ul 13.23 mg/ml YdaT (in 20mM Tris and 200mM NaCl, pH 7.5) mixed with 0.1 ul reservoir solution (0.1M BIS - TRIS pH 5.5, 2.0 M Ammonium sulphate) and equilibrated against 70 ul of reservoir solution |