8BS7

Multimerisation domain of Borna disease virus 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-13
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	34.873, 35.154, 153.125
Unit cell angles	89.97, 90.01, 90.54

Refinement procedure

Resolution	51.040 - 3.200
R-factor	0.3382
Rwork	0.335
R-free	0.38700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.455
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.040	3.314
High resolution limit [Å]	3.200	3.200
Number of reflections	11877	905
<I/σ(I)>	9.9
Completeness [%]	93.2
Redundancy	3.6
CC(1/2)	0.992	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M (NH4)2-tartarate and 20% w/v PEG3350