8BS3

Structure of USP36 in complex with Fubi-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.91883
Spacegroup name	P 21 21 21
Unit cell lengths	63.626, 75.892, 96.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.630 - 2.200
R-factor	0.1972
Rwork	0.196
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	xia2 (3.6.1)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.630	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.177	0.759
Number of reflections	45243	4527
<I/σ(I)>	6	2
Completeness [%]	99.8	99.8
Redundancy	5.1
CC(1/2)	0.985	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M MMT buffer pH 7.0, 25% (w/v) PEG 1500