8BRW
Escherichia coli methionyl-tRNA synthetase mutant L13C,I297C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.810, 45.580, 86.470 |
| Unit cell angles | 90.00, 107.44, 90.00 |
Refinement procedure
| Resolution | 48.770 - 1.730 |
| R-factor | 0.1705 |
| Rwork | 0.169 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h9c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmeas | 0.170 | 1.305 |
| Number of reflections | 60237 | 8415 |
| <I/σ(I)> | 8.1 | 1.39 |
| Completeness [%] | 97.3 | 84.3 |
| Redundancy | 6 | 5.1 |
| CC(1/2) | 0.995 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.08M ammonium citrate, 30 mM potassium phosphate |
