8BRW
Escherichia coli methionyl-tRNA synthetase mutant L13C,I297C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.810, 45.580, 86.470 |
Unit cell angles | 90.00, 107.44, 90.00 |
Refinement procedure
Resolution | 48.770 - 1.730 |
R-factor | 0.1705 |
Rwork | 0.169 |
R-free | 0.20510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h9c |
RMSD bond length | 0.007 |
RMSD bond angle | 0.823 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmeas | 0.170 | 1.305 |
Number of reflections | 60237 | 8415 |
<I/σ(I)> | 8.1 | 1.39 |
Completeness [%] | 97.3 | 84.3 |
Redundancy | 6 | 5.1 |
CC(1/2) | 0.995 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.08M ammonium citrate, 30 mM potassium phosphate |