8BRP
Peptide Arginase OspR from the cyanobacterium Kamptonema sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.290, 154.420, 174.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.19754 |
| Rwork | 0.196 |
| R-free | 0.23317 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.089 | 0.601 |
| Number of reflections | 49464 | 5206 |
| <I/σ(I)> | 15.2 | 3.3 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 200 mM MgAc2, 100 mM Tris-HCl, 25% PEG 3350 |
