8BRO
Structure of the N-terminal domain of BC2L-C lectin (1-131) in complex with a synthetic beta-fucosylamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 63 |
| Unit cell lengths | 43.008, 43.008, 94.217 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.246 - 1.550 |
| Rwork | 0.142 |
| R-free | 0.16870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.250 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.064 | 0.447 |
| Rmeas | 0.076 | 0.540 |
| Rpim | 0.040 | 0.297 |
| Number of reflections | 14192 | 716 |
| <I/σ(I)> | 17.8 | 3.5 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 5.6 | 4.6 |
| CC(1/2) | 0.100 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 292 | 1.2M Tri sodium citrate ph 7.0 |
