8BPD

Structural and Functional Characterization of the Novel Endo-alpha(1,4)-Fucoidanase Mef1 from the Marine Bacterium Muricauda eckloniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 63
Unit cell lengths	105.416, 105.416, 78.247
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.720 - 1.800
R-factor	0.1667
Rwork	0.165
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m8n
RMSD bond length	0.010
RMSD bond angle	1.072
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.720	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.127	1.000
Rmeas	0.137	1.000
Rpim	0.052	0.418
Number of reflections	45842	4540
<I/σ(I)>	6.8	0.48
Completeness [%]	99.9	99.6
Redundancy	6.8
CC(1/2)	0.996	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	24 % PEG 3350, 0.1 M CAPSO pH 9, 3 % isopropanol, 4 % PEG 400