8BP9
Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200W (crystal M200W#2)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Agilent SuperNova |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-10-25 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.974, 74.475, 146.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.400 - 1.700 |
Rwork | 0.193 |
R-free | 0.22840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zal |
RMSD bond length | 0.011 |
RMSD bond angle | 1.598 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.400 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.082 | 0.378 |
Rmeas | 0.094 | 0.455 |
Number of reflections | 61686 | 3153 |
<I/σ(I)> | 9.9 | 2.5 |
Completeness [%] | 99.1 | 97.8 |
Redundancy | 4 | 3.1 |
CC(1/2) | 0.995 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-30% PEG4000, 10-15%PEG400,0.2M MgCl2 in 100 mM Tris-HCl pH 8.5 |