8BOR
Photosensory module from DrBphP without PHY tongue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07225 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 119.630, 64.590, 131.040 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 45.990 - 2.300 |
R-factor | 0.23667 |
Rwork | 0.235 |
R-free | 0.26819 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k5b |
RMSD bond length | 0.004 |
RMSD bond angle | 0.989 |
Data reduction software | XDS (Feb 5, 2021) |
Data scaling software | XSCALE (Feb 5, 2021) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.360 |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.152 | 0.064 | 1.496 |
Rmeas | 0.181 | 0.078 | 1.792 |
Number of reflections | 88343 | 1154 | 6483 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 98.8 | 98.5 | 99.2 |
Redundancy | 3.292 | 3.259 | |
CC(1/2) | 0.989 | 0.993 | 0.325 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 12% polyethylene glycol (PEG) 3350, 200 mM ammonium acetate, 5% fructose, 5% glucose, and 100 mM sodium citrate pH5.6 |