8BOG
Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with Compound 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.570, 106.220, 40.270 |
Unit cell angles | 90.00, 108.51, 90.00 |
Refinement procedure
Resolution | 38.190 - 1.470 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.18000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6q7d |
RMSD bond length | 0.004 |
RMSD bond angle | 0.780 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.190 | 1.560 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmeas | 0.014 | 0.199 |
Number of reflections | 44065 | 7045 |
<I/σ(I)> | 9.79 | 0.87 |
Completeness [%] | 99.6 | 98.3 |
Redundancy | 6.74 | 6.59 |
CC(1/2) | 1.000 | 0.370 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 37,5 % Precipitant Mix 4 (25% (v/v) Hexylene glycol, 25% (w/v) Poly(ethylene glycol) 1000, 25% (w/v) Poly(ethylene glycol) 3350), 0.1M Bis-Tris pH 6.5, 0.1 M Amino acids mix (0.2 M DL-Alanine, 0.2M DL-Glutamic acid monohydrate, 0.2M DL-Lysine monohydrochloride, 0.2M DL-Serine, 0.2M Glycine) |