8BNY

Structure of the tetramerization domain of pLS20 conjugation repressor Rco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	35.328, 36.182, 109.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.356 - 1.429
R-factor	0.234
Rwork	0.233
R-free	0.24910
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.724
Phasing software	Arcimboldo
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.360	1.433
High resolution limit [Å]	1.409	1.409
Rmeas	0.044	0.780
Number of reflections	18842	943
<I/σ(I)>	14.5	1.7
Completeness [%]	91.6
Redundancy	5.1
CC(1/2)	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	0.1 M Hepes pH 7.5, 28% (v/v) PEG 600