8BNB

Crystal structure of Trichoplax Scribble PDZ2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1
Unit cell lengths	32.781, 32.946, 42.710
Unit cell angles	93.63, 95.65, 113.74

Refinement procedure

Resolution	29.970 - 2.110
R-factor	0.2309
Rwork	0.230
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jo7
RMSD bond length	0.002
RMSD bond angle	0.404
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	2.190
High resolution limit [Å]	2.110	2.110
Number of reflections	8998	874
<I/σ(I)>	5.54
Completeness [%]	97.2
Redundancy	3.3
CC(1/2)	0.992	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1.6 M sodium citrate, pH 5.5.