8BNA
BINDING OF HOECHST 33258 TO THE MINOR GROOVE OF B-DNA
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.040, 40.330, 65.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.14 * |
| Refinement software | JACK-LEVITT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 3487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 * | 277 | VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE ACETATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.21 (mM) | |
| 2 | 1 | drop | Hoechst 33258 | 0.21 (mM) | |
| 3 | 1 | drop | 5.38 (mM) | ||
| 4 | 1 | drop | spermine acetate | 0.21 (mM) | |
| 5 | 1 | drop | MPD | 20 (%(w/v)) | |
| 6 | 1 | reservoir | 35 (%) |
