8BMD
Structure of GroEL-ATP complex under continuous turnover conditions
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | JEOL CRYO ARM 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.55 |
Energy Filter | In-column Omega Filter |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.8 |
Software | crYOLO,RELION,PHENIX,CTFFIND,Coot |
Symmetry | D7 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |