8BM0
Structure of GroEL:GroES-ATP complex plunge frozen 200 ms after reaction initiation
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | JEOL CRYO ARM 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.55 |
Energy Filter | In-column Omega Filter |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.4 |
Software | crYOLO,RELION,CTFFIND,Coot,PHENIX |
Symmetry | C7 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |