8BLZ
Engineered Fructosyl Peptide Oxidase - D02 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.096, 129.493, 86.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.750 - 1.700 |
R-factor | 0.16896 |
Rwork | 0.168 |
R-free | 0.19353 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y4j |
RMSD bond length | 0.012 |
RMSD bond angle | 1.723 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.750 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 55830 | 2946 |
<I/σ(I)> | 12.6 | 2.2 |
Completeness [%] | 98.7 | |
Redundancy | 12.7 | |
CC(1/2) | 1.000 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 20000 16-26%, MES 0.1M pH 6.5 |