8BLX
Engineered Fructosyl Peptide Oxidase - X02A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.515, 130.258, 87.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.240 - 1.710 |
R-factor | 0.18262 |
Rwork | 0.181 |
R-free | 0.21035 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y4j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.578 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.682 | 1.739 |
High resolution limit [Å] | 1.710 | 1.710 |
Number of reflections | 55592 | 2685 |
<I/σ(I)> | 12.7 | 1 |
Completeness [%] | 98.9 | |
Redundancy | 11.5 | |
CC(1/2) | 0.998 | 0.461 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 20000 16-26%, MES 0.1M pH 6.5 |