8BLX
Engineered Fructosyl Peptide Oxidase - X02A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.515, 130.258, 87.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.240 - 1.710 |
| R-factor | 0.18262 |
| Rwork | 0.181 |
| R-free | 0.21035 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6y4j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.578 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.682 | 1.739 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Number of reflections | 55592 | 2685 |
| <I/σ(I)> | 12.7 | 1 |
| Completeness [%] | 98.9 | |
| Redundancy | 11.5 | |
| CC(1/2) | 0.998 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 20000 16-26%, MES 0.1M pH 6.5 |
