8BLU
The PDZ domains of human SDCBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.603, 49.588, 118.194 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 63.955 - 1.500 |
| Rwork | 0.183 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n99 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.727 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.070 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 5.446 |
| Rmeas | 0.088 | 5.754 |
| Rpim | 0.024 | 1.840 |
| Number of reflections | 147705 | 7241 |
| <I/σ(I)> | 16.6 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26 | 18.9 |
| CC(1/2) | 1.000 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 50 mM imidazole, 50 mM MES, 100 mM Morpheus amino acid mix, 8.3% PEG 8000, 16.6% Ethylene glycol |
