8BLN
Structure of RutB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.115, 125.107, 123.268 |
| Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
| Resolution | 41.000 - 1.880 |
| R-factor | 0.2527 |
| Rwork | 0.250 |
| R-free | 0.29630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WT model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc2_3793) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.080 | 1.947 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 139409 | 13587 |
| <I/σ(I)> | 7.25 | |
| Completeness [%] | 97.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.995 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG |
