8BLM
Structure of RutB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-18 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.592, 110.890, 128.714 |
| Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
| Resolution | 47.670 - 1.900 |
| R-factor | 0.266 |
| Rwork | 0.263 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.943 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 221505 | 714 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 83.4 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.984 | 0.196 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG |
