8BLE
Structure of GroEL-nucleotide complex in ADP-like conformation plunged 50 ms after mixing with ATP
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Software | crYOLO,RELION,PHENIX,SerialEM,Coot |
| Symmetry | D7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
