8BK5

A structure of the truncated LpMIP with bound inhibitor JK095.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	53.540, 53.540, 77.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.370 - 1.440
Rwork	0.184
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fd9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.920	1.492
High resolution limit [Å]	1.440	1.440
Rmeas	0.060	0.070
Number of reflections	23619	2330
<I/σ(I)>	9.88
Completeness [%]	99.6	98.69
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 %(v/v) 2-propanol, 0.2 M sodium citrate tribasic dihydrate, 0.1 M HEPES, pH 7.5