8BK4
Full length structure of the apo-state LpMIP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.493, 57.529, 67.712 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.830 - 1.340 |
Rwork | 0.197 |
R-free | 0.22050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jvw |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.840 | 1.390 |
High resolution limit [Å] | 1.340 | 1.342 |
Rmeas | 0.080 | 0.100 |
Number of reflections | 28364 | 209 |
<I/σ(I)> | 15.56 | |
Completeness [%] | 74.8 | |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 30 %(v/v) MPD, 0.2 M ammonium acetate, 0.1 M sodium citrate, pH 5.6 |