8BJY
Engineered Fructosyl Peptide Oxidase - X02B mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-30 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.112, 88.493, 90.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.421 - 1.475 |
| Rwork | 0.189 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6y4j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.421 | 1.501 |
| High resolution limit [Å] | 1.475 | 1.475 |
| Rmerge | 0.067 | |
| Rmeas | 0.070 | 0.400 |
| Number of reflections | 82805 | 4255 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 96.0 | 100 |
| Redundancy | 12.7 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 20000 16-26%, MES 0.1M pH 6.5 |
