8BJW
Structure of Tupaia Paramyxovirus C protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07197 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 136.734, 136.734, 63.106 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.460 - 2.390 |
| R-factor | 0.2205 |
| Rwork | 0.219 |
| R-free | 0.25420 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.460 | 67.800 |
| High resolution limit [Å] | 2.390 | 2.590 |
| Rmerge | 0.085 | 0.100 |
| Number of reflections | 27136 | 24104 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | PEG 3350, ammonium citrate dibasic |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | PEG 3350, ammonium citrate dibasic |
