8BJC
Full length structure of the apo-state LpMIP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9673 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.773, 77.773, 103.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.240 - 1.710 |
| R-factor | 0.2418 |
| Rwork | 0.239 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fd9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.240 | 1.772 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.090 | |
| Rmeas | 0.090 | |
| Number of reflections | 19488 | 14883 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 76.7 | 55.53 |
| Redundancy | 12 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 20 %(w/v) PEG 6000, 500 mM zinc acetate dihydrate, 100 mM MES, pH 6.0. |
