8BJC
Full length structure of the apo-state LpMIP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9673 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.773, 77.773, 103.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.240 - 1.710 |
R-factor | 0.2418 |
Rwork | 0.239 |
R-free | 0.28780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fd9 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.962 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.240 | 1.772 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.090 | |
Rmeas | 0.090 | |
Number of reflections | 19488 | 14883 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 76.7 | 55.53 |
Redundancy | 12 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 20 %(w/v) PEG 6000, 500 mM zinc acetate dihydrate, 100 mM MES, pH 6.0. |