8BJ4

Crystal structure of Medicago truncatula histidinol-phosphate aminotransferase (HISN6) in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	74.039, 93.082, 110.518
Unit cell angles	90.00, 106.57, 90.00

Refinement procedure

Resolution	56.430 - 1.450
R-factor	0.1482
Rwork	0.148
R-free	0.18080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MtHISN6_open
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.430	56.430	1.540
High resolution limit [Å]	1.450	4.350	1.450
Rmerge	0.079	0.028	1.359
Rmeas	0.086	0.031	1.475
Total number of observations	1710052
Number of reflections	252775	9588	40508
<I/σ(I)>	13.71	53.46	1.3
Completeness [%]	99.6	99.5	99
Redundancy	6.765	6.736	6.585
CC(1/2)	0.999	0.999	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.2M ammonium acetate; 0.1M BIS_TRIS pH6.5; 25% w/v polyethylene glycol 3,350