8BJ3
Crystal structure of Medicago truncatula histidinol-phosphate aminotransferase (HISN6) in complex with histidinol-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.610, 108.752, 65.973 |
Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
Resolution | 54.380 - 1.610 |
R-factor | 0.1459 |
Rwork | 0.145 |
R-free | 0.17820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MtHISN6_open |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 54.380 | 54.380 | 1.710 |
High resolution limit [Å] | 1.610 | 4.820 | 1.610 |
Rmerge | 0.051 | 0.020 | 0.682 |
Rmeas | 0.058 | 0.022 | 0.788 |
Total number of observations | 405627 | ||
Number of reflections | 98009 | 3753 | 15511 |
<I/σ(I)> | 18.61 | 59.48 | 2.06 |
Completeness [%] | 99.5 | 99.5 | 97.7 |
Redundancy | 4.139 | 4.195 | 3.945 |
CC(1/2) | 0.999 | 0.999 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.2M ammonium acetate; 0.1M BIS_TRIS pH6.5; 25% w/v polyethylene glycol 3,350 |