8BJ1
Crystal structure of Medicago truncatula histidinol-phosphate aminotransferase (HISN6) in the open state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.661, 87.373, 74.235 |
Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
Resolution | 36.970 - 1.570 |
R-factor | 0.1278 |
Rwork | 0.127 |
R-free | 0.16990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3euc |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.970 | 36.970 | 1.660 |
High resolution limit [Å] | 1.570 | 4.700 | 1.570 |
Rmerge | 0.045 | 0.018 | 0.667 |
Rmeas | 0.052 | 0.020 | 0.777 |
Total number of observations | 452887 | ||
Number of reflections | 116740 | 4440 | 18853 |
<I/σ(I)> | 18.02 | 62.37 | 1.86 |
Completeness [%] | 98.6 | 97.9 | 98.6 |
Redundancy | 3.879 | 3.784 | 3.85 |
CC(1/2) | 0.999 | 0.999 | 0.656 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M ammonium sulfate; 0.1 M MES pH 6.5; 30 % w/v PEG 5000 MME |