8BJ0
Crystal structure of Scribble PDZ1 with PTHR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95372 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 82.454, 82.454, 59.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.370 - 2.600 |
R-factor | 0.2775 |
Rwork | 0.277 |
R-free | 0.28520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vwi |
RMSD bond length | 0.002 |
RMSD bond angle | 0.518 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.270 | 2.694 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.082 | |
Number of reflections | 3144 | 313 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 94.7 | |
Redundancy | 3.8 | |
CC(1/2) | 0.998 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M sodium cacodylate pH 5.8, 53% MPD |