8BIA
Crystal structure of Scribble PDZ1 with PTHR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95372 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 53.161, 53.161, 217.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.630 - 2.400 |
R-factor | 0.2529 |
Rwork | 0.252 |
R-free | 0.27130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mtv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.468 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.660 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 6472 | 623 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.4 | 98.9 |
Redundancy | 4.4 | |
CC(1/2) | 0.999 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M phosphate/citrate pH 5.2, 43% PEG 300 |