8BH9

Structure of Est1 from Candida Tropicalis in complex with TLC1 telomerase RNA fragment 427-435 / 496-504

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978880
Spacegroup name	P 21 21 21
Unit cell lengths	36.448, 79.462, 218.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.870 - 2.090
R-factor	0.2072
Rwork	0.206
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.752
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	SHARP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.126	2.122
High resolution limit [Å]	2.086	2.086
Rmerge	0.077	0.927
Rmeas	0.083	0.998
Rpim	0.032	0.366
Number of reflections	37916	1910
<I/σ(I)>	13.8	2.1
Completeness [%]	97.3	100
Redundancy	7.1	7.3
CC(1/2)	0.998	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG 4K 20% PEG 200 5% Tris-HCl 0.1M