8BGL

Structure of the dimeric rsCherryRev1.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97950
Spacegroup name	I 1 2 1
Unit cell lengths	98.104, 48.822, 99.120
Unit cell angles	90.00, 101.16, 90.00

Refinement procedure

Resolution	62.620 - 2.000
R-factor	0.1903
Rwork	0.188
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h5q
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.170	76.170	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.086	0.036	0.613
Rmeas	0.093	0.040	0.667
Rpim	0.036	0.016	0.258
Total number of observations	212739	2312	15091
Number of reflections	31401	387	2300
<I/σ(I)>	12.6	36.9	2.8
Completeness [%]	100.0	99.1	99.9
Redundancy	6.8	6	6.6
CC(1/2)	0.998	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	0.1M MES pH 6.5, 12% w/v PEG 20000