8BGJ

Crystal structure of Reverse Transcriptase domain from Caloramator australicus CART-CAPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 32
Unit cell lengths	96.920, 96.920, 65.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.460 - 1.630
R-factor	0.1846
Rwork	0.183
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ar1
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.940	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.076	1.184
Rmeas	0.092	1.432
Rpim	0.051	0.798
Number of reflections	85450	4204
<I/σ(I)>	9	0.5
Completeness [%]	99.6	99.5
Redundancy	3.1	3.1
CC(1/2)	0.992	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287.15	PEG 3350, Sodium tartrate dibasic dihydrate