8BGF
NMR solution structure of the N-terminal RRM and flanking linker regions of Polypyrimidine tract binding protein 1 using the CYANA CONSENSUS method.
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 900 | |
| 2 | Bruker | AVANCE | 750 | |
| 3 | Bruker | AVANCE | 700 | |
| 4 | Bruker | AVANCE | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 2 | 3D 1H-15N NOESY |
| 17 | 3 | 3 | 15N T2-relaxation-dispersion-CPMG-15NHSQC |
| 18 | 1 | 1 | cross-correlated DD/CSA relaxation-15NHSQC |
| 2 | 1 | 2 | 3D 1H-13C NOESY |
| 3 | 2 | 1 | 15N T1-relaxation-15HSQC |
| 4 | 2 | 1 | 15N T2-relaxation-T1rho-15NHSQC |
| 5 | 2 | 1 | {1H}15N-NOE-15NHSQC |
| 6 | 1 | 1 | relaxation-compensated IzNz Rex HSQC |
| 7 | 1 | 1 | 15N T2-relaxation-dispersion CPMG-15NHSQC |
| 8 | 1 | 1 | 15N T2-relaxation-dispersion-CPMG-15NHSQC |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | 1 | 298 |
| 2 | 6.5 | 1 | 313 |
| 3 | 6.5 | 1 | 293 |
Conformers
| Conformers Calculated Total Number | 2000 |
| Conformers Submitted Total Number | 20 |
