8BGB
Structure of the citrate-bound extracytoplasmic PAS domain of histidine kinase CitA from Geobacillus thermodenitrificans
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.209, 101.140, 77.877 |
| Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
| Resolution | 48.790 - 1.700 |
| Rwork | 0.167 |
| R-free | 0.19580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j80 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.650 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.049 | 0.295 |
| Number of reflections | 105114 | 3571 |
| <I/σ(I)> | 16.47 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 1 M tri-sodium citrate, pH 9.0, 0.1 M imidazole |
