8BFY
ABC transporter binding protein CebE from Streptomyces scabiei in complex with cellotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.390, 39.430, 79.640 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 35.330 - 1.550 |
| Rwork | 0.136 |
| R-free | 0.17760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alphafold model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.373 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.330 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmeas | 0.084 | 1.333 |
| Number of reflections | 53542 | 3849 |
| <I/σ(I)> | 13.15 | |
| Completeness [%] | 99.2 | 97.1 |
| Redundancy | 3.8 | 3.14 |
| CC(1/2) | 0.999 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 277 | PEG3350 25%, sodium citrate pH 3.5 100mM |
