8BFJ
CNOT11-GGNBP2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.322, 51.309, 52.950 |
| Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
| Resolution | 37.060 - 2.230 |
| R-factor | 0.2122 |
| Rwork | 0.209 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.512 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.060 | 2.486 |
| High resolution limit [Å] | 2.230 | 2.400 |
| Number of reflections | 17833 | 760 |
| <I/σ(I)> | 12.55 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.992 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 1.5 M ammonium sulfate 1.5M, MPD 5% (w/v), 0.05 M Tris pH 8.0 |
