8BF4
Crystal structure of Mouse Plexin-B1 (20-535) in complex with VHH15 and VHH14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-06 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873127 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.267, 109.233, 95.478 |
Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
Resolution | 95.260 - 2.150 |
R-factor | 0.2118 |
Rwork | 0.211 |
R-free | 0.22780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8bb7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.801 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 95.653 | 95.470 | 2.540 |
High resolution limit [Å] | 2.149 | 11.890 | 2.480 |
Rmerge | 0.088 | 0.045 | 0.679 |
Rmeas | 0.121 | 0.063 | 0.938 |
Rpim | 0.084 | 0.045 | 0.645 |
Number of reflections | 76707 | 663 | 4482 |
<I/σ(I)> | 8.3 | ||
Completeness [%] | 99.9 | ||
Redundancy | 3.4 | 3 | 3.4 |
CC(1/2) | 0.984 | 0.925 | 0.366 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% (w/v) PEG 6000, 0.1 M sodium citrate, pH 5.5, 3% dextran sulphate (Mr 5000) |