8BDK
Structure of a non-canonical histone from archaea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.016, 81.154, 53.543 |
Unit cell angles | 90.00, 98.71, 90.00 |
Refinement procedure
Resolution | 52.925 - 1.880 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b67 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.764 |
Data reduction software | xia2 |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.910 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.099 | |
Rpim | 0.053 | 0.670 |
Number of reflections | 28933 | 1466 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 100.0 | |
Redundancy | 3.3 | |
CC(1/2) | 0.996 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M NaOAc pH 4.6, 10% (w/v) PEG 3350 and 5% (w/v) tacsimate pH 4.6 |