8BD9
Crystal structure of TRIM33 alpha PHD-Bromo domain in complex with 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.953, 79.953, 134.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.680 - 3.200 |
| R-factor | 0.23895 |
| Rwork | 0.232 |
| R-free | 0.34854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u5m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.950 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.066 | 0.545 |
| Rmeas | 0.077 | 0.625 |
| Rpim | 0.037 | 0.294 |
| Number of reflections | 4598 | 633 |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.8 | 4.1 |
| CC(1/2) | 0.999 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.2 M calcium chloride and 20 % wt/vol PEG-3350 |
