8BD8
Crystal structure of TRIM33 alpha PHD-Bromo domain in complex with 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 80.015, 80.015, 134.469 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.300 - 3.100 |
| R-factor | 0.21502 |
| Rwork | 0.210 |
| R-free | 0.33826 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u5m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.679 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.470 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.160 | 1.115 |
| Rmeas | 0.171 | 1.186 |
| Rpim | 0.059 | 0.398 |
| Number of reflections | 4853 | 692 |
| <I/σ(I)> | 11 | 2.3 |
| Completeness [%] | 97.1 | |
| Redundancy | 7.9 | |
| CC(1/2) | 0.997 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 0.2 M calcium chloride and 20 % wt/vol PEG-3350 |
