8BD1

Crystal structure of the RhsPWHH-RhsPI toxin-immunity pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9790
Spacegroup name	P 41 21 2
Unit cell lengths	51.580, 51.580, 206.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.790 - 1.260
R-factor	0.1989
Rwork	0.198
R-free	0.21860
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.790	1.310
High resolution limit [Å]	1.260	1.260
Number of reflections	76480	7488
<I/σ(I)>	22.38
Completeness [%]	100.0
Redundancy	2
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.4M potassium sodium tartrate and 19% PEG3350