8BCL
Recombinant Tipula oleracea Nudivirus polyhedrin Expanded unit cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-04 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.5498 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.146, 57.146, 106.044 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.870 - 2.800 |
| R-factor | 0.2409 |
| Rwork | 0.239 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8bc5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.760 |
| Data reduction software | xia2.multiplex |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 4961 | 478 |
| <I/σ(I)> | 6.39 | |
| Completeness [%] | 93.8 | |
| Redundancy | 3 | |
| CC(1/2) | 0.955 | 0.282 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 301 | protein crystallises in the cell |
